Aivia 2D
Application-Specific Solutions for 2D and 2D+T Images

What You Get

Aivia Base

The backbone of all Aivia packages. It features high performance volume and surface rendering engines capable of handling even the most demanding (multi-terabyte, multi-channel time series) microscopy datasets. It offers a wide range of visualization models which enables you to explore your data. Basic quantitative options such as Measurement, Spreadsheet, Charts and Annotations are also included with Aivia Base.

2D Object Detection + Tracking

Aivia 2D includes several analysis pipelines called recipes for tracking objects (e.g. cells, nuclei, particles and filaments). Each recipe uses advanced image processing algorithm specific to the application for object enhancement and detection. The recipes detect objects of interest on your image and track their motility over time.

Machine Learning Pixel Classifier

You can train a machine learning model to distinguish objects of interest from background. The Pixel Classifier provides a powerful way to detect objects in images where conventional threshold-based methods are not enough.

Track and Lineage Editing

Edit tracking analysis results using the Track and Lineage Editor. It features a simple single-button control scheme that allows you to drag-and-drop track points to connect tracks, disconnect tracks and create lineages. All the changes are updated automatically in measurements.


The Aivia 2D package is for researchers looking to analyze 2D and 2D+time datasets acquired in fluorescence or phase contrast microscopy. The analysis recipes in Aivia applies state-of-the-art image processing algorithm to enhance, detect and track objects (cells, nuclei, particles, etc.) automatically and can be easily adjusted to your datasets. You also have access to a wide range of morphological, intensity, motion and lineage measurements with the recipes for comprehensive analysis of your data.

Features at-a-glance
  • Display and work with huge (100s GBs) datasets
  • Complete analysis pipelines for 2D object detection and tracking
  • Robust image processing algorithm that works for many typical imaging conditions
  • Detect 2D objects (e.g. cells, nuclei, organelles, particles, etc.)
  • Track object lineages, including cell division and cell fate
  • Access a wide range of morphology, intensity and motion measurements
  • Two (2) years of technical support plus software updates included


Aivia's image analysis solutions automatically calculate many measurements for all detected objects as well as statistics about the entire population. You can explore the data further using the built-in Charts in Aivia and classify objects of interest using our machine learning object classifier tool.

Here are some common measurements that Aivia provides:

Object Outlines
  • Area

  • Perimeter

  • Length

  • Width

  • Intensity

  • Position (x, y)

  • Circularity

  • Count

Motility and Lineage
  • Total Time

  • Velocity

  • Acceleration

  • Lineage Length

  • Current Lineage Generation


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