Open top light sheet microscope. In Press: A.K. Glaser et al., Nature Communications (2019) Click for pre-print.
Thy1-EGFP labeled mouse cortex processed with PEGASOS 2 tissue clearing method is imaged on 3i's Cleared Tissue Lightsheet system.
Isotropic live imaging of organotypic neuron
Thy1-EGFP mouse brain processed with second generation PEGASOS method (PEGASOS 2) developed by Hu Zhao at Texas A&M University
Hippocampus neurons of H-line mouse cleared with RapiClear 1.52 and imaged on Nikon A1R, 488nm, APOTIRF60x/1.49 oil
Bright field imaged cells plus deep learning predicted dapi (nuclei) localization. (NIBIB/NIH, Bethesda, USA)
Fluorescently labelled mitochondria in cell imaged by iSIM. Image enhanced by deep learning restoration. (NIBIB/NIH, Bethesda, USA) (NIBIB/NIH, Bethesda, USA)
University of California - Santa Barbara Live imaging of drosophila embryogenesis
University of California San Francisco - Parnassus
High magnification micrograph of a sinonasal papilloma, also Schneiderian papilloma and Schneiderian polyp. H&E stain
Time lapse recording showing the development of neuron polarity in a hippocampal neuron growing in vitro. This recording captures not only the formation of the axon (the longest process, oriented downward in the last frame), but also accelerated development and outgrowth of the dendritic arbor due to treatment with BMP-7, a growth factor that has been found to selectively promote dendritic development in these cells [as described in Withers et al., (2000) PMID: 10651865].
Histopathology of invasive ductal carcinoma of the breast representing a scirrhous growth. Core needle biopsy. HER-2/neu oncoprotein expression by Ventana immunostaining system
D.F. Pace, A.V. Dalca, T. Geva, A.J. Powell, M.H. Moghari, P. Golland, “Interactive whole-heart segmentation in congenital heart disease”, Medical Image Computing and Computer Assisted Interventions (MICCAI 2015), Lecture Notes in Computer Science; 9351:80-88, 2015.
King's College, London UK
Meyerowitz lab, California Institute of Technology
M. sexta antennal lobe combined
Benchmark data from: Stegmaier, J.; Arz, J.; Schott, B.; Otte, J. C.; Kobitski, A.; Nienhaus, G. U.; Strähle, U.; Sanders, P. & Mikut, R.: "Generating Semi-Synthetic Validation Benchmarks for Embryomics", Proc., IEEE International Symposium on Biomedical Imaging: From Nano to Macro, 2016. Stegmaier, J., Mikut, R.: "Fuzzy-based Propagation of Prior Knowledge to Improve Large-Scale Image Analysis Pipelines", PLOS ONE, 2017.
Gladstone Institute, San Francisco, CA
Image courtesy of Prof. Gail McConnell, University of Strathclyde. The data set shows a newly emerged female Drosophila imago stained with propidium iodide only. This image is composed by 242 optical sections taken with an axial separation of 6.3 um, forming a z‐stack 1.53 mm deep. The image was taken using a Mesolens confocal microsocope.
Developing Drosophila Melanogaster embryo. Raw image from Dr. Keller. Detection and Tracking done in Aivia 6.1. Microscope: SIMView light-sheet microscope Objective lens: 16X/0.8 (water) Pixel size (microns): 0.406 x 0.406 (x 2.03) Time step (sec): 30
Arborization by Daniel Bloodgood is licensed under a Creative Commons Attribution 4.0 International License. https://creativecommons.org/licenses/by/4.0/
Data from Deciphering the Mechanisms of Developmental Disorders (https:/dmdd.org.uk), a programme funded by the Wellcome Trust with support from the Francis Crick Institute, is licensed under a Creative Commons Attribution licence.
Bobby Kasthuri, Ph.D. under the tutelage of Jeff Lichtman, M.D., Ph.D., acquired a data set from mouse cortex with a 3x3x30 cubic nanometer spatial resolution, yielding 660GB of images. This beautiful data was published Friday, July 30th 2015 in Cell.
Maximum intensity projection of Golgi impregnated pyramidal neurons in the neocortex of an adult mouse, imaged via serial block-face scanning electron microscopy. Contrast is reversed so that Golgi stain appears bright against the unstained background. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.