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Aivia 11

Accelerate Your Analysis with AI
Deep Learning Object Segmentation

Leverage the power of AI with integrated Cellpose for robust and reproducible object detection

Multi-well Experiments

Explore and analyze data from entire experiments in Aivia and export to CytoMAP for additional analyses


Customize your image analysis workflow by combining Aivia's AI tools and recipes for efficient batch image analysis

DL Cell Segmentation
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Deep Learning Object Segmentation

Complete object detection powered by Cellpose

Aivia 11 incorporates state-of-the-art deep learning models by Cellpose (Stringer, 2021) [1] for object detection. We are offering AI access for all with four new Cellpose-integrated analysis recipes for 2D and 3D object segmentations - no knowledge of Python scripting or training deep learning models necessary.

The models are highly adaptable to various imaging modalities and staining conditions. Whether your imaging experiment involves DIC systems or fluorescent membrane stains, the object segmentation recipes powered by Cellpose enable you to get robust and reproducible segmentation every time.

  • Use Cellpose's powerful object detection deep learning models
  • No knowledge of Python scripting or deep learning model training needed
  • Leverage existing Aivia tools for your analysis routine

In Aivia 9, we were the first commercially-available software to offer AI-powered object segmentation with Cellpose by the way of Python scripts. By integrating Cellpose into our existing infrastructure, Aivia 11 makes it easier to deploy deep learning cell segmentation in your workflow and get results up to 80% faster.

[1] Stringer C, Wang T, Michaelos M, Pachitariu M. Cellpose: a generalist algorithm for cellular segmentation. Nature Methods. 18: 100-106. (2021)

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Multi-well Experiments

Multi-well Experiments

Analyze your entire experiment

Your experiments can have multiple test conditions at the same time and your analysis software should let you analyze them all at once. In Aivia 11, we are introducing several features that allow you to visualize, explore, and analyze your multi-well experiments and accelerate insights creation on a single platform.


Starting with your imaging experiment, Aivia 11's new Experiment Explorer allows you to import Leica multi-well data into Aivia. You can explore images from individual wells or imaging experiments from the Multi-well Navigator and assign labels to annotate the experiment conditions for your well plates.

  • Import and explore your entire multi-well experiment with intuitive Navigator
  • Define experimental conditions for downstream analysis
  • Export results to CytoMAP for further data exploration
  • Support for Leica's multi-well data format from the MICA imaging system and more

You can analyze your image using Aivia's built-in tools on a single FOV and build an analysis pipeline using the new Workflows tool. The analysis workflow you built can then be batch applied to your entire imaging experiment - or just on select wells with your annotated conditions.

The analysis results collected from batch applying the workflow can be exported to CytoMAP (Stoltzfus, 2020) [2] for further data exploration. Leverage CytoMAP's capabilities (such as dimension reduction) to accelerate insight creation from your imaging experiment, speeding up your route to publication.

[2] Stoltzfus CR, Filipek J, Gern BH, et al. CytoMAP: a spatial analysis toolbox reveals features of myeloid cell organization in lymphoid tissues. Cell reports. 31(3): 107523.

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Complete analysis pipeline

We are giving you greater freedom than ever to customize your image analysis workflow in Aivia 11. The Workflow Processor allows you to build and customize complete analysis pipelines from data import to object classification and export.

The Workflow Processor allows you to incorporate all the analysis tools available in Aivia - image enhancement functions, AI-powered Pixel Classifier, deep learning models, image analysis recipes, measurements, and Object Classifier - into a single workflow. Additionally, you can set up multiple workflows to run successively to provide you with even more analysis capabilities.

  • Mix and match Aivia's tools to create your own analysis pipeline
  • Workflows can be built from scratch or from previously-analyzed results
  • Batch apply your customized workflow for quick results

The process for building an analysis workflow is integrated with the tools in Aivia. The 'Create a Workflow' feature loads the recipe image enhancements, and Pixel Classifier you used for image analysis into the Workflow Processor automatically - giving you a quick way to create a batch routine to apply your analysis to an entire experiment. This eliminates the need to recall each analysis step you took so you can focus on understanding your results.

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Additional improvements

  • Faster mesh preview for 3D Cell Analysis and 3D Cellpose recipes

  • Create 2D and 3D objects from labeled images

  • AiviaWeb with permanent storage and automatic archiving available as a subscription option

Additional Improvements

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