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CHO cells nuclei virtual staining - BioImage.io



Author

Estibaliz Gómez de Mariscal, Mario Del Rosario


Description

A conditional cycleGAN trained to infer the cell membrane as seen in a widefield fluorescence image from phase contrast images of CHO cells imaged with phase contrast microscopy. The cells are imaged with a 20x objective.


  • Input channel: 2D images, grayscale

  • Bit depth: 32-bit float

  • Output channels: Cell membrane probability


This model was downloaded and converted from BioImage.io, respecting the associated license (see License section below for more information)


Download

By downloading, installing, copying, accessing, or using the software, you agree to the terms of this end user license agreement.


Download model file and test image (ZIP archive)



Requirements

Make sure you have installed Aivia and the required DeepLearning module (according to our Wiki).


Installation and apply instructions

Aivia is required for applying the model file. You can request a demo copy of Aivia here.

  1. Drag-and-drop the model file into the Recipe Console area; or use the 'Load recipe' option in the Recipe Console to load the model file.

  2. Load the test image (or any image of your own) into Aivia.

  3. If your image contains more than one channel, click on the 'Input & Output' section and specify the image channel you wish to apply the model on.

  4. Click 'Start' to apply the model.


License

Copyright 2025 de Mariscal, MIT. Full license information can be found here.


Acknowledgement

Bioimage.io is supported by AI4Life. AI4Life has received funding from the European Union's Horizon Europe research and innovation program under grant agreement number 101057970.



References

Isola et al. arXiv in 2016

Lucas von Chamier et al. biorXiv 2020

 
 
 

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