ImageJ-Fiji Quick Tip #2: Easily annotating videos
Updated: Jun 16, 2020
Showing a video with annotations is really efficient for lab meetings, conference talks. Here is a note to show you how you can quickly and easily annotate a video / time-lapse acquisition with ImageJ - Fiji software…
Give Me the Protocol
Open ImageJ - Fiji and open your time-lapse video
Choose a tool to make your annotation (e.g. the oval tool)
Create one annotation and then press “t” on your keyboard
Move to the next image and move/modify your annotation and press again “t”
Once you have finished, click on "More > Options" in the ROI Manager window and tick the option "Associate Show All ROIs with slices" if not active
Then Image > Overlay > From ROI Manager
You can then save your video as .avi (File > Save As...)
Note: not satisfied with the result? Delete all annotations with "Image > Overlay > Remove Overlay"...